The present task aims to quantify the ratio of synaptic vesicles proteins in rat brain fractions. The enrichment of vesicular proteins is performed using rat brain homogenate subjected to differential centrifugation (Huttner et al., 1983) leading to synaptosoms (P2) and crude synaptic vesicles (LP2) fractions. VGLUT1 (65kDa) and Syp (38kDa) are visualized using specific antibodies and the ECL detection system. After developing bands are scanned using a flatbed scanner or a gel documentation machine for further quantification.

Charité – Universitätsmedizin of Humboldt University, Berlin
Centre of Anatomy, Functional Cell Biology
Johannes-Friedrich Zander
Phillipstr. 12, 10115 Berlin

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